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DTSTART;TZID=Europe/Amsterdam:20260428T143000
DTEND;TZID=Europe/Amsterdam:20260428T160000
DTSTAMP:20260625T133027
CREATED:20260413T092125Z
LAST-MODIFIED:20260413T092125Z
UID:5536-1777386600-1777392000@graduateschool-eps.info
SUMMARY:BIC Seminar: Prof. Rainer Hedrich
DESCRIPTION:BIC Seminar: Prof. Rainer Hedrich \nProbing plant signalling via Channelrhodopsin optogenetics \nDate: 28 April 2026 \nTime: 14:50 \nLocation: C0333\, Orion\, Wageningen Campus \nAbstract: \nMembrane electrical excitation\, often associated with the transient rise in cytosolic Ca2+ and H+ concentration\, represent universal signalling elements that couple a wide range of stimuli to their characteristic responses\, including in plants. Despite decades of intensive research\, many of the signal transduction elements involved in Ca2+ and pH signalling are yet to be elucidated. Progress has been hampered due to our inability to trigger defined changes in cytosolic Ca2+ concentration\, without provoking changes in plasma membrane electrical properties. Over the past 5 years\, new game-changing ion channel optogenetic tools have been introduced into plants to answer long-standing questions in plant biology. \nIn this presentation I will describe how we have engineered the light-gated ChannelRhodopsins CHRs selective for anions (ACR1)\, H+ (KCR2) or Ca2+ (XXM2.0) and to obtain stable expression in Arabidopsis and tobacco plants. These tools provide us with the unique opportunity to non-invasively induce voltage\, Ca2+ and H+ signals in guard cells as well as any plant cell/tissue/organ type of interest. Furthermore\, I will describe how CHRs provide a valuable tool to non-invasively excite the plasma membrane\, trigger H+ or Ca2+ influx\, and provoke H+ or Ca2+ induced Ca2+ release. This allows to study the interrelation between plant pH and Ca2+ signalling on one side\, and electrical excitation on the other. Depending on the CHR and stimulation features (pulse number\, length\, and frequency) one can remote control single cell action\, long-distance transport as well as growth and shape or plant organs. Our findings challenge established paradigms relating to the way proton\, calcium\, and voltage signalling are interconnected in plants and rethink of mechanisms involved.
URL:https://graduateschool-eps.info/event/bic-seminar-prof-rainer-hedrich/
LOCATION:Wageningen Campus\, Droevendaalsesteeg 4\, Wageningen\, Gelderland\, 6708 PB\, Netherlands
CATEGORIES:EPS courses,Seminars
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